Responses of proline, lipid peroxidation and antioxidative enzymes in two varieties of Pisum sativum L. under salt stress

Document Type : Research Paper


1 Biochemistry Lab, Centre for Rural Development and Technology, Indian Institute of Technology, Hauz Khas, New Delhi 110016, India.

2 Centre for Energy Studies, Indian Institute of Technology, Hauz Khas, New Delhi 110016, India.

3 Plant Molecular Biology Lab. (ICGEB) Aruna Asaf Ali Marg, New Delhi-110067, India.

4 Department of Botany, Faculty of Science, Hamdard University, New Delhi 110062, India.


The possible involvement of activated oxygen species in the mechanism of damage by NaCl
stress was studied in leaves of two varieties of pea (Pisum sativum L.) cv. EC 33866 and Puget. The
level of lipid peroxidation, enzyme activity of superoxide dismutase (SOD, EC, ascorbate
peroxidase (APX, EC, glutathione reductase (GR, EC, dihydroascorbate reductase
(DHAR, were studied. High chlorophyll stability was observed in EC 33866 varieties than
Puget. Significant accumulation in the levels of Na+ and Cl- was observed in Puget as the
concentration of salt increased. Decrease in levels of K+, Ca2+ and Mg2+ was recorded in both
varieties but Puget showed more decrease as compared to EC 33866. Salt stress increased the rate of
lipid peroxidation in both the varieties of pea but more decrease was observed in Puget. Salt stress
induced significant increase in the activities of the antioxidant enzymes. The SOD and APX increased
in both varieties at all concentrations but the increase was more in EC 33866 variety than Puget. GR
and DHAR were increased in both the varieties at 150 mM NaCl, however at 200 mM NaCl decline
was observed. Decline of DHAR and GR was more pronounced in Puget as compared to the EC
33866 variety.