Effect of plant-derived smoke extract on in vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas) microspore-derived embryos

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

2 Department of Plant Breeding, Tarbiat Modares University, Tehran, Iran.

Abstract

The effect of aqueous smoke extract, derived from Tanacetum parthenium, on
in vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas)
microspore-derived embryos (MDEs) was evaluated in this study. Inoculation of
rapeseed MDEs with the smoke-extract dilutions (1:250, 1:500, 1:1000 and 1:2000)
for various times (5, 10 and 15 min) significantly enhanced the percentage of
regenerated plantlets from these embryos and also decreased the callogenesis
percentage in these explants. The root length and shoot length of inoculated MDEs
were significantly improved with all smoke-extract dilutions except for dilutions of
1:100 and 1:10. Application of smoke-extract solutions (1:1000, 1:500, 1:250 and
1:100) in B5 regeneration medium significantly enhanced the plantlet regeneration
from rapeseed MDEs compared to control cultures. Furthermore, use of smokeextract
solutions in combination with GA3 (0.1 and 0.15 mgl-1) significantly
improved the root length and shoot length of rapeseed MDEs compared to separate
use of smoke-extract solutions. Use of filter paper saturated with aqueous smoke
extract (1:250) on top of the B5 regeneration medium was the best smoke treatment
method improving the plantlet regeneration percentage, root length and shoot
length of rapeseed MDEs compared to other treatment methods. The conversion
frequency of rapeseed MDEs pretreated with smoke extract dilution of 1:500 was
improved up to 50% compared with the control MDEs (26.67%), whereas the use
of smoke extract (1:100) in B5 regeneration medium enhanced the conversion
frequency of these MDEs up to 62% compared with the control cultures (

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