1Gorgan University of Agricultural Sciences and Natural Resources, Iran.
2Agricultural Research and Education Organization, Iran.
Hybrids as various resources have valuable importance in tree breeding. Today, improvement programs by using poplar and willows and their hybrids play basic role in supplying wood and decreasing pressure on natural forests. This research was carried out in order to study inter-generic hybridization possibility between Salix aegyptica and Populus caspica species from Salicaceae to achieve new hybrids. Embryo rescue technique was used in order to produce new inter-generic hybrids in salicaceae. The experiment was performed in factorial completely randomized design with 3 replication in embryo developmental stages and different media treatments. The analysis of data showed that inter-generic cross ability between Salix aegyptica and Populus caspica was possible and 14 day after pollination and MS medium containing 3 percent sucrose were the best time and medium for obtaining high amount of germinated hybrids.