TY - JOUR ID - 657 TI - Bulblet production from node explant grown in vitro in hybrid lilies JO - International Journal of Plant Production JA - IJPP LA - en SN - 1735-6814 AU - Kapoor, R. AU - Kumar, S. AU - Kanwar, J.K. AD - Biotechnology Department, University of Horticulture & Forestry, Solan (H.P.), India. Y1 - 2012 PY - 2012 VL - 3 IS - 4 SP - 1 EP - 6 KW - Asiatic hybrids KW - Bulblet KW - growth regulators KW - Micropropagation KW - Node explant KW - Oriental hybrids DO - 10.22069/ijpp.2012.657 N2 - An attempt was made to regenerate bulblets from ex vitro node explants of hybrid lilies in the year 2007. Node sections (3-4 mm) isolated from the middle part of the stem in hybrid lilies were cultured on Murashige and Skoog (MS) medium supplemented with several combinations of NAA and BA. Growth regulator-free medium was not effective in inducing bulblet regeneration. A significant increase in the percent of explant producing bulblets and number of bulblets per explant was observed when 2 mg/l NAA was used in combination with 1.5 or 2 mg/l BA. The heaviest bulblets were obtained with 2 mg/l NAA in combination with 1.5 mg/l BA after 90 days of culture. The cultivar Apeldoorn produced greater number of bulblets whereas more weighty bulblets were produced in the cultivar Beartix. 1 or 2 mg/l IBA was most effective in producing roots. The rooted bulblets were hardened with 80-82% survival success after 30 days of transfer in the pots. UR - https://ijpp.gau.ac.ir/article_657.html L1 - https://ijpp.gau.ac.ir/article_657_9c2201f4b5d928f73a07d94a858df26c.pdf ER -